The third daughter of Martin Segeš Komenský and his wife Anna, sister of Comenius.
František Vyskočil hypothesises that after the death of her parents in 1605, Markéta went to her older sister Zuzana (and that for the same reason Jan went to his aunt Zuzana Nohálová at Strážnice). Markéta Komenská was the second wife of Pavel Bozkovský, a burgher of Uherský Brod. After his death (in 1622) she married Martin Kožur, bailiff at Havřice in 1627.